The MAPK Slt2/Mpk1 plays a role in iron homeostasis through direct regulation of the transcription factor Aft1

Iron is an essential element for life. Cells develop mechanisms to tightly regulate its homeostasis, in order avoid abnormal accumulation and the consequent cell toxicity. In budding yeast, high affinity iron regulon under control of transcription factor Aft1. We present evidence demonstrating that MAPK Slt2 wall integrity pathway (CWI), phosphorylates negatively regulates Aft1 activity upon depletion signal, both fermentative or respiratory conditions. The lack provokes dysfunction leading a shorter chronological life span. signal scarcity not transmitted through other signalling pathways such as TOR1 , PKA, SNF1 TOR2 / YPK1 . observation physically binds rather suggests direct regulation. interacts with modulate transcriptional function on “iron regulon”. Upon activation transcription, phosphorylation by occurs contributing downregulate This regulatory mechanism relevant extension. • Slt2/Mpk1 response deprivation participates feed-back over metabolic conditions dysregulation slt2 strain shortens span does require binding Grx3/Grx4 high-affinity